Coding

Part:BBa_K2950002:Design

Designed by: Shuxin Xiao   Group: iGEM19_BNDS_China   (2019-10-16)


Toluene dioxygenase(TodC1C2)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
    Illegal BglII site found at 714
    Illegal BglII site found at 729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1527
    Illegal NgoMIV site found at 82
    Illegal NgoMIV site found at 1384
    Illegal NgoMIV site found at 1905
    Illegal NgoMIV site found at 1947
    Illegal AgeI site found at 1511
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our design, TodC1C2 is utilized to oxidize hexadecylbenzene to the intermediate cis-hexadecylbenzene dihydrodiol.


Source

Pseudomonas putida

References